charge-coupled device digital camera sony dkc-5000  (Sony)

Journal:

Article Title: Detection on Surfaces and in Caco-2 Cells of Campylobacter jejuni Cells Transformed with New gfp, yfp , and cfp Marker Plasmids

doi:

Figure Lengend Snippet: Fluorescence of C. jejuni RM1221 transformed with Pc-gfp, Pc-yfp or Pc-cfp fusion plasmids. RM1221 was transformed with either pWM1007 (1221gfp), pWM1008 (1221yfp), or pWM1009 (1221cfp). (A) Colonies of 1221gfp, 1221yfp, or 1221cfp. All three strains were grown on BA-KM amended with bacteriological charcoal. Colonies were photographed using a Leica MZ-FLIII fluorescence stereomicroscope equipped with appropriate filters and a Sony DKC-5000 CCD camera. (B) Individual 1221gfp, 1221yfp, or 1221cfp cells visualized using the gfp, yfp, and cfp filter sets. Cells were visualized using a Leica DM-RB epifluorescence microscope equipped with a Sony DKC-5000 CCD camera.

Article Snippet: Bacterial colonies were photographed using a Leica MZ-FLIII fluorescence stereomicroscope equipped with a DKC-5000 charge-coupled device (CCD) camera (Sony Medical Systems, Park Ridge, N.J.); individual cells were photographed using a Leica DM-RB epifluorescence microscope (Leica Microsystems) equipped with a Sony DKC-5000 CCD camera.

Techniques: Fluorescence, Transformation Assay, Microscopy

Journal:

Article Title: Detection on Surfaces and in Caco-2 Cells of Campylobacter jejuni Cells Transformed with New gfp, yfp , and cfp Marker Plasmids

doi:

Figure Lengend Snippet: Representative microscopic images of yellow and cyan C. jejuni colonies and cells following inoculation of chicken breast skin or after growth in vitro. (A, B, and D) Colonies were photographed using a Leica MZ-FLIII fluorescence stereomicroscope equipped with the appropriate filters and a Sony DKC-5000 CCD camera. Individual cells (C) were visualized using a Leica DM-RB epifluorescence microscope equipped with a Hamamatsu C4742-95 cooled CCD camera (Hamamatsu Photonics K.K., Hamamatsu, Japan). Panels A, B, and D and the right half of panel C represent a composite of the same field photographed with the yfp and cfp filter sets. The image obtained with the yfp filter set was overlaid onto the image obtained with the cfp filter set using Corel Photo-Paint9 (Corel Corp., Ottawa, Canada). (A and B) Colonies of 1221cfp and 781yfp (A) or 1221yfp and 781cfp (B). Cells were inoculated onto a sample of chicken skin, removed, and grown on CCDA amended with 200 μg of kanamycin per ml. (C) Bright-field (left half) and epifluorescent (right half) images of 781yfp and 1221cfp aggregates. Mixtures (1:1) of 781yfp and 1221cfp cells were grown for 24 h in BB, vortexed vigorously, and observed as a wet mount. The aggregates in the left half of panel C are not the same as those in the right half. (D) Colonies of 1221cfp and 781yfp. Cells were grown for 24 h in BB-KM and plated on BA-KM amended with 0.4% bacteriological charcoal.

Article Snippet: Bacterial colonies were photographed using a Leica MZ-FLIII fluorescence stereomicroscope equipped with a DKC-5000 charge-coupled device (CCD) camera (Sony Medical Systems, Park Ridge, N.J.); individual cells were photographed using a Leica DM-RB epifluorescence microscope (Leica Microsystems) equipped with a Sony DKC-5000 CCD camera.

Techniques: In Vitro, Fluorescence, Microscopy